UVAS Library catalog › Results of search for 'an,phr: 5743' with limit(s): 'su-to:Department of Microbiology'

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Abid Sarwar

Dr.Aftab Ahmad Anjum

Prof. Dr. Azhar

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Prof.Dr.Khushi Muhammad

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	1.	No cover image available Sero-Prevalence Of Brucellosis In Buffaloes And Cattle Of Swat Valley And Government Livestock Farms,Nwfp by Azhar Khan \| Prof.Dr.Masood Rabbani \| Prof.Dr.Azhar \| Prof.Dr.Khushi Muhammad \| Faculty of Veterinary Sciences. Material type: Book; Format: print Publisher: 2008Dissertation note: Brucellosis is an infectious zoonotic disease that is associated with chronic debilitating infections in humans and reproductive failure in domestic animals (Corbell, 1997). The sero-prevalence of brucellosis in buffaloes and cattle were undertaken by collecting samples from animals brought to various slaughterhouses and Private and Govt. farms in Swat valley and Peshawar division by screening through i-LLISA, MRT and RBPT. Out of 850 samples, 600 sera, 200 milk samples were collected along with 50 samples of slaughterhouse worker, butcher and veterinarian for this study. All the serum samples tested through RBPT and I-ELISA showed the overall prevalence 3.67% and 4.33% in the cattle and buffaloes population respectively while the combined prevalence in the cattle, buffaloes and human population through RBPT was 3.38 % and through i-ELISA was 4%. The high rate of brucellosis was recorded through RBPT and i-ELISA in buffaloes ( 4.75%,5.5%) while 0.0% prevalence in male buffaloes through RBPT and iELISA, where as in female buffaloes it was 4.85% through RBPT and through i-ELISA was 5.626%. The comparatively low rate (1.5%) of brucellosis was noted in cattle through the RBPT and 2% through i-ELISA while in female cattle it was 1.587% through the RBPT and through i-ELISA 2.12% with 0.0% in males. Among the serum samples (30) of buffalo and cattle having reproductive disorder were tested through the same tests which showed overall prevalence 16.6%. The prevalence at Cattle Breeding and Dairy farm 1-larichand and Livestock Research and Development Farm (Surrezai was 0.0% through Milk Ring Test and i-ELISA. Also cattle milk samples (110) from private farms in swat valley showed prevalence through Milk Ring Test 0.9% and through i-ELISA prevalence was noted to be 1 .82%. As 50 human serum samples were tested through RBPT and i-ELISA but none of these samples were positive showed that the prevalence of brucellosis in human being is very low, The comparison of RBPT, MRT and i-ELISA (milk and serum) was also analyzed statistically by z-test, the data revealed insignificant results. Availability: Items available for loan: UVAS Library [Call number: 1012,T] (1). Place hold Add to cart (remove)
	2.	No cover image available Comparative Efficicy Of Peste Des Petits Ruminant (Ppr) Vaccine S Available In Pakistan In Sheep And Goats by Muhammad Intizar \| Prof.Dr.Manur-Din-Ahmad \| Dr.Aftab Ahmad Anjum \| Prof.Dr.Azhar \| Faculty of Veterinary Sciences. Material type: Book; Format: print ; Nature of contents: ; Literary form: Publisher: 2008Dissertation note: The present study was designed to evaluate the physical factors affecting the PPR 'vaccine and also to compare the efficacy of the locally available PPR vaccines in Pakistan in sheep and goats. The current study was conducted on 120 different small ruminants ((60 sheep and goats). The humoral immune response was monitored by measuring the antibodies titre through Hl and AGID test. HI and AGID test are reliable and effective methods of diagnosing viral diseases and to evaluate the humoral immune response. It was concluded that the vaccine should he stored either at -20°C or at 4 C.The vaccine stored at 27 C had a drop in HA titre and no HA activity was found at 40 C To evaluate the HA activity of PPR virus it is better to use chicken or human group'O' R.B.Cs in a concentration of 1%. The diluent should have the pH 6.8- 7.0. By evaluating the vaccine efficacy in sheep it was found that after 14th day of vaccination there was a gradual increase in the antibodies titer till the 56th day of vaccination. The locally manufactured vaccine was having a geometric mean titre (GMT)207.9 while the (GMT) of Pestivec was 73.3. 63d day post vaccination. In goats the locally manufactured vaccine was having a geometric mean titer (GMT) 147. while the (GMT) of Pestivec was 48.5.63rd day post vaccination. No antibodies production was there in any control group. It was concluded form the study that locally produced vaccine is equally good and could be used confidently. It will save also helpful in saving the foreign reserves oh the country. Availability: Items available for loan: UVAS Library [Call number: 1051,T] (1). Place hold Add to cart (remove)
	3.	No cover image available Role Of Herbal Polysaccharides As Immunomodulator by Muhammed Zafar \| Dr.Aftab Anjum \| Dr.Muhammed Imran Najeeb \| Prof.Dr.Azhar. Material type: Book; Format: print Publisher: 2009Dissertation note: Compared to the birds in vaccinated groups that were kept without feeding Livol, the sera of the NDV vaccinated birds kept on Livol had higher antibody titers on day 42.amongst various treatment groups the highest Haemagluttination-Inhibtion titers was recorded in group C feed with Livol treated birds as compared to the other groups. The hematological parameters observed i.e. Packed Cell Volume, Hemoglobin and Total Leukocyte Count were higher in group C2 and C3 than in Group Cl, which shows that increasing Livol concentration have increased the above said parameters. The higher concentrations of Livol (Herbal Polysaccharides) have increased body weight gain than the birds fed with low concentrations of Livol (Herbal Polysaccharides). Treatment related changes in body weight, organ body weight ratio of thymus; spleen and Bursa of fabricius were also observed amongst the various groups. The addition of Livol (Herbal Polysaccharides feed to diminished the adverse/immunosuppressive effects of different vaccine on antibody titers against Newcastle and most relative organ weights. These findings suggested that Livol (Herbal Polysaccharide) can effectively stimulate/enhance the body weight gain, immunity in broiler chicks and Livol (Herbal Polysaccharides) can be potential ameliorator against various vaccines and its adverse/suppressive effects in broiler chicks. Availability: Items available for loan: UVAS Library [Call number: 1065,T] (1). Place hold Add to cart (remove)
	4.	No cover image available Antigenic Characterisation Of H9 Subtype Avian Influenza Viruses Isolated Desi And Zoo Birds by Farrukh Saleem \| Dr.Muhammad Mahmood Mukhtar \| Prof.Dr.Azhar \| Prof.Dr.Khushi Muhammad \| Faculty of Veterinary Sciences. Material type: Book; Format: print ; Literary form: not fiction Publisher: 2009Dissertation note: Avian influenza is a viral infection which affects mainly the respiratory system of birds. The H7N3 subtype influenza viruses were isolated for the first time in 1994 from breeder flock in northern areas of Pakistan. A second wave of avian influenza outbreak was detected in 1999. The causative agent of this outbreak was H9N2. The H9N2 considered as low pathogenic avian influenza (LPAI) virus and continuously circulating in poultry flocks causing enormous economic losses to poultry industry of Pakistan. This showed that avian influenza viruses are present in commercial poultry. Most of the efforts to isolate avian influenza A viruses are from commercial poultry and these isolations are outbreak based. That is why we have mad’ an effon to isolate and identify H9 subtype avian influenza viruses from apparently healthy live desi and zoo birds (Lahore, Pakistan). We have successfully isolate H9 subtype influenza viruses from these birds during our study. As these viruses have RNA genome and their RNA polymerase enzyme lacks proof reading activity which resulted in spontaneous mutation in surface glycoproteins (HA and NA) and reassortment of their genomic segments results in escape from host immune response produced by the vaccine. This is the reason that every year we require a new candidate virus for vaccine preparation. We have made an effort to isolate and identify avian influenza viruses from live desi and zoo birds of Lahore and performed antigenic characterization. In this way, we have been able to know the exact status of avian influenza virus strains present in the desi and zoo birds. We also have seen that the imported vaccine have less interaction with the local strains and gives less protective titer although it gives best titers when we raise antisera against imported vaccine. The local vaccines although gives a little bit less titer when we raise the antisera against these vaccines but their antisera have more interaction with the local H9 subtype antigen so it gives better protective immune response. By this study we have seen that antisera obtained from infected chicken give more antibody titer as compare to antibody raised in the rabbits. Infected chicken antisera are more reactive as compare to rabbit antisera. This shows that our isolates have highest similarity with the currently circulating viruses. All above results helped us to devise a new control strategy against avian influenza viral infections present in these local birds. The antigenic characterization of these avian influenza isolates helped us to see the antigenic differences between the isolates of this study and H9 subtype avian influenza viruses used in vaccines. Therefore, this study clearly suggests that a new local H9 subtype avian influenza virus should be used as vaccinal candidate every year for the effective control of influenza viral infections of poultry. Availability: Items available for loan: UVAS Library [Call number: 1082,T] (1). Place hold Add to cart (remove)
	5.	No cover image available Microbial Evaluation Of Raw Meat At Abattoirs And Retail Outlests (Lahore) by Abid Sarwar \| Prof. Dr. Mansur ud Din Ahmad \| Dr. Imran Najeeb \| Prof. Dr. Azhar. Material type: Book; Format: print Publisher: 2010Dissertation note: The objective of this study was to evaluate the microbial quality of meat. The present study was planed to determine the aerobic plate count on meat obtained from the abattoirs and local market. A total of 90 meat samples that were collected for determining the microbiological quality of meat. Half of the meat samples (n=45) were collected from various abattoirs and half of the meat samples (n=45) were collected from retail outlets in Lahore City to get an idea of contamination from slaughtering point to retail outlets. These samples were processed for Aerobic plate counts, E.coli, S.aureus and Salmonella counts. Overall, this study revealed that the level of contamination on meat carcasses was higher in retail meat shops compared to the abattoir. However, the microbial contamination in the abattoir were high if we compare these results to the reports from developing countries like India, Iran and Bangladesh. Bacterial isolates identified and counted from this study were Staphylococcus aureus (44) out of 90 samples was the most abundant as 48.88%, followed by E. coli (43) 47.77% and Salmonella (26) 28.88%. Statistical analysis revealed that analysis of variance between various abattoir and the retail meat shops for E.coli, Salmonella and S.aureus showed significant differences with some exceptions. E.coli counts were significantly higher (P < 0.05) in the meat shops and abattoirs. For E.coli most of the data were significant at 5% level (P < 0.05) with some exception in case of beef and goat samples taken from abattoirs which were non significant because of the unhygienic environments. Analysis of variance for Salmonella between various abattoir and the retail outlets were significant at 5% level (P < 0.05). For S.aureus between various abattoir and the retail outlets showed non significant at 5% level (P > 0.05) with some exceptions in case of beef abattoir and goat retail outlet samples taken which were significant at 5% level (P < 0.05). The higher incidence of microbial load in fresh meat obtained in this study might be attributed to unhygienic and improper handling of animals during slaughter, dressing, evisceration, transportation and unhygienic environments at the retail shops. The usual practice of washing the carcass with the same water in which intestines and offal had been washed was considered as one of the predominant reasons for increased microbial counts of the carcasses. A complete ignorance on the part of the meat handlers/ butchers in hygienic handling of carcasses during slaughter and retailing processes might be the main factors for producing meat with high microbial load. Levels of microbial contamination in Pakistani abattoirs and traditional retail meat shops reflect the hygiene status of meat production in the developing world. Education of the meat retailers' community which runs the traditional meat shops, in terms of the importance of hygienic and sanitary precautions would go a long way towards providing wholesome and safe meat to the consumers. Availability: Items available for loan: UVAS Library [Call number: 1196,T] (1). Place hold Add to cart (remove)